Effect of Magnetic Field on Adhesion of Muscle Cells to Culture Plate
Shigehiro Hashimoto, Keiji Tachibana
The effect of a magnetic field on adhesion of cultured muscle
cells to the culture plate has been studied in vitro. An
experimental system was manufactured to apply a magnetic
field to muscle cell culture. The system consists of a couple of
solenoid coils, a culture dish of 52 mm internal diameter, and an
inverted phase-contrast microscope. The solenoid coil
generates the alternating magnetic field of 13 mT of the
effective value at a period of 0.01 s with the electric current of
the rectangular pulses. C2C12 (Mouse myoblast cell line
originated with cross-striated muscle of C3H mouse) cells were
suspended in Dulbecco’s Modified Eagle’s Medium. The
suspension was poured into the plastic dish placed on the stage
of the microscope. The culture dish was exposed to the
magnetic field between the solenoid coils at 29 degrees Celsius.
For comparative study, a part of the suspension was poured into
the same kind of dish without exposure to the magnetic field at
29 degrees Celsius. The number of cells, which adhered to the
bottom of the culture dish, was traced according to the time
(<130 min) during exposure to the alternating magnetic field.
The experimental results show that adhesion is accelerated with
alternating magnetic field of 13 mT. Full Text
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